xiap polyclonal antibody  (Proteintech)

Journal: Cell reports

Article Title: The Hao-Fountain syndrome protein USP7 regulates neuronal connectivity in the brain via a novel p53-independent ubiquitin signaling pathway

doi: 10.1016/j.celrep.2025.115231

Figure Lengend Snippet:

Article Snippet: Rabbit anti-Xiap , Bioss Antibodies , Cat# bs-1281 R, RRID:AB_10856518.

Techniques: Western Blot, Immunoprecipitation, Immunofluorescence, Virus, Recombinant, Protease Inhibitor, Magnetic Beads, Mass Spectrometry, Software

Journal: PLOS One

Article Title: Altered Bcl-2/Caspase signaling and hypoxia-induced apoptosis in primary human aniridia limbal stromal cells, in CoCl 2 mediated hypoxic stress, in vitro

doi: 10.1371/journal.pone.0328157

Figure Lengend Snippet: CASP3, CASP7, CASP8, CASP9 and CASP10 mRNA levels (A-E), BCL2, BAX and BID mRNA levels (F-H) and CDKN1A, CDKN1B, TNFα, XIAP and BIRC5 mRNA levels (I-M) are shown. Values are expressed on a logarithmic scale (Log 2) with geometric mean±geometric standard deviation. Two-way ANOVA followed by Dunnett’s test was used, significant p values are indicated. BCL2 mRNA levels were significantly higher in AN-LSCs compared to LSCs (p = 0.0291). In LSCs, BCL2 mRNA level significantly decreased (p = 0.0102) and CDKN1B level significantly increased using 75 µM CoCl 2 treatment. In addition, in AN-LFCs, 75 µM CoCl₂ treatment significantly decreased CASP3, CASP9 and BCL2 mRNA levels (p = 0.0494; p = 0.0414; p = 0.0218).

Article Snippet: XIAP Polyclonal antibody , XIAP , Proteintech , #23453–1-AP , Rabbit , 1:100.

Techniques: Standard Deviation

Journal: PLOS One

Article Title: Altered Bcl-2/Caspase signaling and hypoxia-induced apoptosis in primary human aniridia limbal stromal cells, in CoCl 2 mediated hypoxic stress, in vitro

doi: 10.1371/journal.pone.0328157

Figure Lengend Snippet: Data represent mean±SD from three independent experiments. Two-way ANOVA followed by Dunnett’s test was used, significant p values are indicated. p21, p27, XIAP, TNF-α, and Survivin protein levels are shown. Representative histograms show primary antibody staining in green, while staining with the corresponding secondary antibody alone (negative control) is shown in red. MFI: mean fluorescence intensity, normalized to the secondary antibody control. In AN-LSCs, p21 and p27 protein levels were significantly higher (p = 0.0003; p = 0.0164) and XIAP and Survivin protein levels were significantly lower (p = 0.0003; p = 0.0074), than in LSCs, using flow cytometry. In addition, using ELISA, in cell culture supernatant of AN-LSCs, TNF-α protein levels were significantly higher following 75 µM CoCl 2 treatment, than in untreated controls (p = 0.0251).

Article Snippet: XIAP Polyclonal antibody , XIAP , Proteintech , #23453–1-AP , Rabbit , 1:100.

Techniques: Staining, Negative Control, Fluorescence, Control, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: Cell proliferation

Article Title: Elevated COMMD1 Contributes to Cardiomyocyte Copper Efflux in Chronic Myocardial Ischemia: Insights From Rhesus Monkey.

doi: 10.1111/cpr.70016

Figure Lengend Snippet: FIGURE 3 | Increased XIAP associated with COMMD1 in cardiomyocytes after chronic LAD ligation in rhesus monkeys. (A) The mRNA levels of COMMD1 determined by RT-PCR. (B) Western blot and (C) quantitative analysis of the protein levels of p300 and XIAP in different portions of the heart of rhesus monkey (Sham: n = 4; MI: n = 4). (D) Immunofluorescence image and (E) co-localization analysis showing COMMD1 (green) or XIAP (green) with cardiomyocytes (α-actinin, red) in the serial sections of the heart of rhesus monkey, DAPI stain (blue) labels nuclei, scale bar = 100 μm. (F) Immunoprecipitation-Western blotting analysis of COMMD1 and XIAP in the ischemic heart tissue of rhesus monkeys and the control res- in + mouse antibody against COMMD1 or coupling resin + mouse IgG as the control groups. (G) Western blotting analysis of the protein levels of COMMD1 and XIAP in the cardiac tissues of rhesus monkeys after 2 and 24 h of LAD ligation (Sham: n = 4; 2 h MI: n = 2; 24 h MI: n = 2). (H) The ratio of XIAP versus COMMD1 protein levels in the heart of rhesus monkeys. Data were expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus Sham control. IA, infarct area; RA, remote area.

Article Snippet: PVDF membranes were incubated overnight at 4°C with the respective primary antibodies: monoclonal mouse anti- CCS (1:100, SC- 55560, Santa cruz, USA), monoclonal mouse anti- COMMD1 (1:100, SC- 166248, Santa cruz, USA), polyclonal rabbit anti- COMMD1 (1:1000, A5174, Abclonal, China), monoclonal mouse anti- XIAP (1:100, SC- 55551, Santa Cruz, USA), polyclonal rabbit anti- XIAP (1:1000, NB- 100- 56,183, Novus, USA), monoclonal rabbit antiATP7A (1:1000, ab308524, abcam, USA), monoclonal rabbit anti- ATP7B (1:1000, ab131208, abcam, USA), monoclonal mouse anti- GAPDH (1:1000, TA- 08, Zhongshan, China) and monoclonal rabbit anti- p300 (1:1000, 86,377, CST, USA).

Techniques: Ligation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunofluorescence, Staining, Immunoprecipitation, Control

Journal: Cell proliferation

Article Title: Elevated COMMD1 Contributes to Cardiomyocyte Copper Efflux in Chronic Myocardial Ischemia: Insights From Rhesus Monkey.

doi: 10.1111/cpr.70016

Figure Lengend Snippet: FIGURE 4 | Effect of XIAP overexpression on COMMD1 levels in H9C2 cells under varying copper concentrations. (A) Western blotting analy- sis of the protein levels of COMMD1 and XIAP after 48 h of XIAP-overexpression-plasmid transfection. (B) Quantitative analysis of the mRNA and protein levels of XIAP and (C) COMMD1 after 48 h of XIAP-overexpression-plasmid transfection. (D) Outline for the treatment of H9C2 cells. (E) Western blot and quantitative analysis of the protein levels of COMMD1 after 48 h of transfection with XIAP-overexpression-plasmid and treatment with 50 μM TEPA. (F) Western blot and quantitative analysis of the protein levels of COMMD1 after 48 h of transfection with XIAP-overexpression- plasmid and treatment with 50 μM CuSO4. Data (n = 3) were expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus mismatch group.

Article Snippet: PVDF membranes were incubated overnight at 4°C with the respective primary antibodies: monoclonal mouse anti- CCS (1:100, SC- 55560, Santa cruz, USA), monoclonal mouse anti- COMMD1 (1:100, SC- 166248, Santa cruz, USA), polyclonal rabbit anti- COMMD1 (1:1000, A5174, Abclonal, China), monoclonal mouse anti- XIAP (1:100, SC- 55551, Santa Cruz, USA), polyclonal rabbit anti- XIAP (1:1000, NB- 100- 56,183, Novus, USA), monoclonal rabbit antiATP7A (1:1000, ab308524, abcam, USA), monoclonal rabbit anti- ATP7B (1:1000, ab131208, abcam, USA), monoclonal mouse anti- GAPDH (1:1000, TA- 08, Zhongshan, China) and monoclonal rabbit anti- p300 (1:1000, 86,377, CST, USA).

Techniques: Over Expression, Western Blot, Plasmid Preparation, Transfection

Journal: Cell proliferation

Article Title: Elevated COMMD1 Contributes to Cardiomyocyte Copper Efflux in Chronic Myocardial Ischemia: Insights From Rhesus Monkey.

doi: 10.1111/cpr.70016

Figure Lengend Snippet: SCHEME 1 | Elevated COMMD1 contributes to cardiomyocyte-copper efflux during chronic ischemic hearts of rhesus monkeys, regulated by XIAP through post-translational modification.

Article Snippet: PVDF membranes were incubated overnight at 4°C with the respective primary antibodies: monoclonal mouse anti- CCS (1:100, SC- 55560, Santa cruz, USA), monoclonal mouse anti- COMMD1 (1:100, SC- 166248, Santa cruz, USA), polyclonal rabbit anti- COMMD1 (1:1000, A5174, Abclonal, China), monoclonal mouse anti- XIAP (1:100, SC- 55551, Santa Cruz, USA), polyclonal rabbit anti- XIAP (1:1000, NB- 100- 56,183, Novus, USA), monoclonal rabbit antiATP7A (1:1000, ab308524, abcam, USA), monoclonal rabbit anti- ATP7B (1:1000, ab131208, abcam, USA), monoclonal mouse anti- GAPDH (1:1000, TA- 08, Zhongshan, China) and monoclonal rabbit anti- p300 (1:1000, 86,377, CST, USA).

Techniques: Modification

Journal: Molecular Therapy. Nucleic Acids

Article Title: HBx induces chemoresistance in diffuse large B cell lymphoma by inhibiting intrinsic apoptosis via the NF-κB/XIAP pathway

doi: 10.1016/j.omtn.2024.102346

Figure Lengend Snippet: XIAP-mediated apoptosis is associated with resistance to EPI and VCR in HBx-positive DLBCL cells (A and B) Relative mRNA expression of multidrug resistance molecules (A) and IAP family members (cIAP1, cIAP2, XIAP, Survivin, and Livin) (B) in DLBCL cells of each group. (C) Western blotting for XIAP protein expression. (D) Flow assay and quantification plots of the effects of EPI and VCR on apoptosis of GFP-SUDHL-4, HBx-SUDHL-4, GFP-U2932, and HBx-U2932 cells after knockdown XIAP. Data are presented as the mean ± SEM. The results were determined in triplicate, and the error bars represent the mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: The following antibodies were used: anti-HBX (bs-2147R, Bioss), XIAP (bs-1281R, Bioss), cleaved caspase-3 (341034, Zenbio), cleaved PARP-1 (380374, Zenbio), glyceraldehyde 3-phosphate dehydrogenase (bs-2188R, Bioss), NF-κB p65 (FNabo06089, FineTest), p65(Ser536) (HY-P80839, MCE), CD20 (no. 48750, CST), and PAX5 (no. 12709, CST).

Techniques: Expressing, Western Blot, Knockdown

Journal: Molecular Therapy. Nucleic Acids

Article Title: HBx induces chemoresistance in diffuse large B cell lymphoma by inhibiting intrinsic apoptosis via the NF-κB/XIAP pathway

doi: 10.1016/j.omtn.2024.102346

Figure Lengend Snippet: H&E staining and immunohistochemical staining of tumor tissues in the chemotherapy group detecting the expression of XIAP, cleaved caspase-3, and cleaved PARP-1

Article Snippet: The following antibodies were used: anti-HBX (bs-2147R, Bioss), XIAP (bs-1281R, Bioss), cleaved caspase-3 (341034, Zenbio), cleaved PARP-1 (380374, Zenbio), glyceraldehyde 3-phosphate dehydrogenase (bs-2188R, Bioss), NF-κB p65 (FNabo06089, FineTest), p65(Ser536) (HY-P80839, MCE), CD20 (no. 48750, CST), and PAX5 (no. 12709, CST).

Techniques: Staining, Immunohistochemical staining, Expressing

Journal: Molecular Therapy. Nucleic Acids

Article Title: HBx induces chemoresistance in diffuse large B cell lymphoma by inhibiting intrinsic apoptosis via the NF-κB/XIAP pathway

doi: 10.1016/j.omtn.2024.102346

Figure Lengend Snippet: HBx-mediated NF-κB hyperactivation upregulates XIAP in vitro (A) Relative expression of transcription factors NF-κB p65, CREB1, Sp1, C/EBPα, and P53 mRNAs in each group of DLBCL cells. (B) Effect of transfection of NF-κB p65 and CREB1 plasmids on the expression of XIAP. (C) Dual-luciferase reporter gene assay for the impact of transcription factors NF-κB p65 and CREB1 on XIAP promoter activity. (D and E) Western blotting to detect total NF-κB p65, phosphorylated NF-κB p65 (D), and the nuclear and cytoplasmic NF-κB p65 protein (E). (F) Semiquantitative analysis of the nuclear-to-cytoplasmic ratio of NF-κB p65 protein. The results were determined in triplicate, and the error bars represent the mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01.

Article Snippet: The following antibodies were used: anti-HBX (bs-2147R, Bioss), XIAP (bs-1281R, Bioss), cleaved caspase-3 (341034, Zenbio), cleaved PARP-1 (380374, Zenbio), glyceraldehyde 3-phosphate dehydrogenase (bs-2188R, Bioss), NF-κB p65 (FNabo06089, FineTest), p65(Ser536) (HY-P80839, MCE), CD20 (no. 48750, CST), and PAX5 (no. 12709, CST).

Techniques: In Vitro, Expressing, Transfection, Luciferase, Reporter Gene Assay, Activity Assay, Western Blot

Journal: Molecular Therapy. Nucleic Acids

Article Title: HBx induces chemoresistance in diffuse large B cell lymphoma by inhibiting intrinsic apoptosis via the NF-κB/XIAP pathway

doi: 10.1016/j.omtn.2024.102346

Figure Lengend Snippet: HBx-induced XIAP upregulation is closely related to NF-κB p65 nuclear translocation (A) Immunofluorescence staining to observe the expression and location of NF-κB p65 proteins in each group of DLBCL cells (200×). (B and C) Effect of different concentrations of BAY 11–7082 on XIAP mRNA and protein in HBx-SUDHL-4 and HBx-U2932 cells, respectively.

Article Snippet: The following antibodies were used: anti-HBX (bs-2147R, Bioss), XIAP (bs-1281R, Bioss), cleaved caspase-3 (341034, Zenbio), cleaved PARP-1 (380374, Zenbio), glyceraldehyde 3-phosphate dehydrogenase (bs-2188R, Bioss), NF-κB p65 (FNabo06089, FineTest), p65(Ser536) (HY-P80839, MCE), CD20 (no. 48750, CST), and PAX5 (no. 12709, CST).

Techniques: Translocation Assay, Immunofluorescence, Staining, Expressing

Journal: Molecular Therapy. Nucleic Acids

Article Title: HBx induces chemoresistance in diffuse large B cell lymphoma by inhibiting intrinsic apoptosis via the NF-κB/XIAP pathway

doi: 10.1016/j.omtn.2024.102346

Figure Lengend Snippet: HBx induces chemoresistance in DLBCL by inhibiting intrinsic apoptosis via the NF-κB/XIAP pathway

Article Snippet: The following antibodies were used: anti-HBX (bs-2147R, Bioss), XIAP (bs-1281R, Bioss), cleaved caspase-3 (341034, Zenbio), cleaved PARP-1 (380374, Zenbio), glyceraldehyde 3-phosphate dehydrogenase (bs-2188R, Bioss), NF-κB p65 (FNabo06089, FineTest), p65(Ser536) (HY-P80839, MCE), CD20 (no. 48750, CST), and PAX5 (no. 12709, CST).

Techniques: